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KMID : 1041219920340010041
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Journal of Sericultural and Entomological Science
1992 Volume.34 No. 1 p.41 ~ p.48
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Degradation of the Parasporal Crystal Proteins of Bacillus thuringiensis in Gut Juice of Some Insects
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金英勳/Kim, Young Hoon
金尙賢/姜錫權/Kim, Sang Hyun/Kang, Seok Kwon
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Abstract
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Dissolution and degradation of the parasporal crystal proteins produced from B. thurimgiensis var. kurstaki B. thuringiensis, var. dendrolimus and B. thurimgiensis var. aizawai were investigated. SDS-polyacrylamide gel electrophoresis analysis showed that the crystals contained major protein with molecular weight of approximately 134 kDa for B. thurimgiensis var. aizawai, 143 kDa for B. thurimgiensis var. kurstaki and 149 kDa for B. thurimgiensis var. dendrolomus, respectively. Crystals of three other strains were incubated alkali solutions at various pH or gut juice of Silkworm Bombyx mori, Fall webworm Hyphantria cunea. and Common Cabbage worm Pieris rapae. When crystals of these strains were solubilized by alkali solutions, no major differences among strains B. thurimgiensis could be detected. Among the strains studied, crystal proteins (130~66 kDa) consist of protease resistant polypeptides in the 45 ~66 kDa size range when treated with gut juice of three insect species.
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